The accuracy and precision of the presented method were corroborated by low intra- and inter-day variations in quantitative results in all plant part extracts. Flavan-3-ol monomers (+)-catechin and (−) -epicatechin were found in all plant parts but their procyanidin dimers were exclusively identified in the stem and root. The leaf extract contained high concentrations of chlorogenic acid (∼100 µg/mg extract) and a variety of quercetin glycosides that were also detected in the fruit and stem extracts. Using a metabolomics-based approach for compound identification with an emphasis on phenolic metabolites, a single HPLC-PAD-APCI/MS method was developed for the separation and quantitation of the major metabolites found in the 95% ethanol extracts of leaf, stem, root and fruit. While the anthocyanin content of the fruit has been well characterized, the chemistry of the vegetative parts used in supportive therapy for diabetes has been largely ignored. (Lowbush blueberry), which is a widely used natural health product, particularly for the treatment of diabetic symptoms.
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